"
Team:Calgary/5 August 2010
From 2010.igem.org
| Line 8: | Line 8: | ||
| - | <u> Raida </u> | + | <u>Raida</u> |
Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes. | Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes. | ||
| Line 21: | Line 21: | ||
I did a miniprep of the plasmid switched pRFP overnights then I did a PCR of plasmid as well as a restriction digest using EcoRI and SpeI. Then I ran the 0.8% gel at 90V. The gel failed as all that was amplified was an empty plasmid. I will try to restrict again using all NEB enzymes and buffers because we ran out of invitrogen halfway. In order to get more product I am doing a gradient PCR of pRFP again and will try to PCR purify tomorrow using the vacuum method. I also designed the biography page on the wiki today. | I did a miniprep of the plasmid switched pRFP overnights then I did a PCR of plasmid as well as a restriction digest using EcoRI and SpeI. Then I ran the 0.8% gel at 90V. The gel failed as all that was amplified was an empty plasmid. I will try to restrict again using all NEB enzymes and buffers because we ran out of invitrogen halfway. In order to get more product I am doing a gradient PCR of pRFP again and will try to PCR purify tomorrow using the vacuum method. I also designed the biography page on the wiki today. | ||
| + | |||
| + | |||
| + | <u>Patrick</u> | ||
| + | |||
| + | Finished the basic preliminary structure of the wiki's front page. Will fix up some links and streamline the code tomorrow. Then it's off to start the Team page. | ||
}} | }} | ||
Revision as of 22:37, 6 August 2010
Thursday August 5, 2010
Dev
OBTAINED COLONIES! Six colonies from each of the four plates were chosen to be tested for the correct construct (K135000+I13504 or K135000+I13507) by means of a colony PCR.
Raida
Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes.
Chris
Today, I spent the day contacting sponsors and preparing the sponsorship letters that would be used to contact the different faculties. I found colonies of the pSB1AC3 and pSB1AK3 from the registry and made overnight cultures to be Miniprepped tomorrow. Finally, I began the digest of the CpxP promoter in preparation of inserting it into a plasmid.
Jeremy
I did a miniprep of the plasmid switched pRFP overnights then I did a PCR of plasmid as well as a restriction digest using EcoRI and SpeI. Then I ran the 0.8% gel at 90V. The gel failed as all that was amplified was an empty plasmid. I will try to restrict again using all NEB enzymes and buffers because we ran out of invitrogen halfway. In order to get more product I am doing a gradient PCR of pRFP again and will try to PCR purify tomorrow using the vacuum method. I also designed the biography page on the wiki today.
Patrick
Finished the basic preliminary structure of the wiki's front page. Will fix up some links and streamline the code tomorrow. Then it's off to start the Team page.
No notebook page exists for this date. Sorry!
