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Team:UNIPV-Pavia/Material Methods/Instruments
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==AUTOCLAVE== | ==AUTOCLAVE== | ||
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An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121°C or more, typically for 15 to 20 minutes depending on the size of the load and the contents. It was invented by Charles Chamberland in 1879, although a precursor known as the <i>steam digester</i> was created by Denis Papin in 1679. The name comes from Greek <i>auto</i>, ultimately meaning <i>self</i>, and Latin <i>clavis</i> meaning <i>key</i> — a self-locking device. | An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121°C or more, typically for 15 to 20 minutes depending on the size of the load and the contents. It was invented by Charles Chamberland in 1879, although a precursor known as the <i>steam digester</i> was created by Denis Papin in 1679. The name comes from Greek <i>auto</i>, ultimately meaning <i>self</i>, and Latin <i>clavis</i> meaning <i>key</i> — a self-locking device. | ||
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| [[Image:UNIPV_Pavia_autoclave.jpg | 250px |right|Autoclave]] | | [[Image:UNIPV_Pavia_autoclave.jpg | 250px |right|Autoclave]] | ||
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==BACTERIA ROOM== | ==BACTERIA ROOM== | ||
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This is a room in which we work with bacteria. The temperature in this room is very hot and it smells like puke. | This is a room in which we work with bacteria. The temperature in this room is very hot and it smells like puke. | ||
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| [[Image:UNIPV_Pavia_bacteriaroom.jpg | 250px |right|Bacteria room]] | | [[Image:UNIPV_Pavia_bacteriaroom.jpg | 250px |right|Bacteria room]] | ||
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==INCUBATOR FOR PLATES== | ==INCUBATOR FOR PLATES== | ||
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This is a oven in which plates are kept at 37 C to grow bacteria. | This is a oven in which plates are kept at 37 C to grow bacteria. | ||
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| [[Image:UNIPV_Pavia_incubatorforplates.jpg | 250px |right|Incubator for plates]] | | [[Image:UNIPV_Pavia_incubatorforplates.jpg | 250px |right|Incubator for plates]] | ||
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==INCUBATOR AND SHAKER FOR LIQUID CULTURES== | ==INCUBATOR AND SHAKER FOR LIQUID CULTURES== | ||
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This is a oven where bacteria are kept to grow. In this case you can define temperature an rpm. | This is a oven where bacteria are kept to grow. In this case you can define temperature an rpm. | ||
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| [[Image:UNIPV_Pavia_incubatorshaker.jpg | 250px |right|Incubator and shaker for liquid cultures]] | | [[Image:UNIPV_Pavia_incubatorshaker.jpg | 250px |right|Incubator and shaker for liquid cultures]] | ||
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==MOLECULAR BIOLOGY ROOM== | ==MOLECULAR BIOLOGY ROOM== | ||
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This is the room in which we treat DNA. | This is the room in which we treat DNA. | ||
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| [[Image:UNIPV_Pavia_molbioroom.jpg | 250px |right|Molecular biology room room]] | | [[Image:UNIPV_Pavia_molbioroom.jpg | 250px |right|Molecular biology room room]] | ||
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==HOOD== | ==HOOD== | ||
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It is a laminar flow hood for handling highly toxic products. | It is a laminar flow hood for handling highly toxic products. | ||
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| [[Image:UNIPV_Pavia_hood.jpg | 250px |right|Hood]] | | [[Image:UNIPV_Pavia_hood.jpg | 250px |right|Hood]] | ||
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==WATER BATH== | ==WATER BATH== | ||
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It is a bath in which water is heated to high temperatures for gel extraction and heath shock during bacterial transformation. | It is a bath in which water is heated to high temperatures for gel extraction and heath shock during bacterial transformation. | ||
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| [[Image:UNIPV_Pavia_waterbath.jpg | 250px |right|Water bath]] | | [[Image:UNIPV_Pavia_waterbath.jpg | 250px |right|Water bath]] | ||
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==INSTRUMENTS FOR ELECTROPHORESIS== | ==INSTRUMENTS FOR ELECTROPHORESIS== | ||
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Gel electrophoresis apparatus - Agarose gel is placed in this TBE buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is the black one while the positive is the red one. In this way DNA, that is naturally negatively charged, migrates to the positive pole. | Gel electrophoresis apparatus - Agarose gel is placed in this TBE buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is the black one while the positive is the red one. In this way DNA, that is naturally negatively charged, migrates to the positive pole. | ||
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| [[Image:UNIPV_Pavia_electrophoresis.jpg | 250px |right|Instruments for electrophoresis]] | | [[Image:UNIPV_Pavia_electrophoresis.jpg | 250px |right|Instruments for electrophoresis]] | ||
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==GEL IMAGE ACQUISITION SYSTEM== | ==GEL IMAGE ACQUISITION SYSTEM== | ||
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It is a UV system with camera used to acquire gel run snapshots. | It is a UV system with camera used to acquire gel run snapshots. | ||
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| [[Image:UNIPV_Pavia_gelacq.jpg | 250px |right|Gel image acquisition system]] | | [[Image:UNIPV_Pavia_gelacq.jpg | 250px |right|Gel image acquisition system]] | ||
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==FREEZER ROOM== | ==FREEZER ROOM== | ||
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This is a room where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria. | This is a room where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria. | ||
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| [[Image:UNIPV_Pavia_freezer.jpg | 250px |right|Freezer room]] | | [[Image:UNIPV_Pavia_freezer.jpg | 250px |right|Freezer room]] | ||
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==FRIDGE ROOM== | ==FRIDGE ROOM== | ||
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This is a room in which we have a fridge to stock plates and LB medium | This is a room in which we have a fridge to stock plates and LB medium | ||
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| [[Image:UNIPV_Pavia_fridge.jpg | 250px |right|Fridge room]] | | [[Image:UNIPV_Pavia_fridge.jpg | 250px |right|Fridge room]] | ||
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==TECAN Infinite 2000== | ==TECAN Infinite 2000== | ||
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Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. | Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. | ||
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| [[Image:UNIPV_Pavia_tecan.jpg | 250px |right|TECAN Infinite 2000]] | | [[Image:UNIPV_Pavia_tecan.jpg | 250px |right|TECAN Infinite 2000]] | ||
Revision as of 07:31, 6 August 2010
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