Team:UNIPV-Pavia/Calendar/August

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Revision as of 15:55, 5 August 2010

AUGUST

DATE	ACTIVITY
August, 1st	44° Bio-Lab - I20 and I21 plates showed colonies. Some were picked, inoculated and grown in proper medium.
AUGUST: WEEK 1
August, 2nd	45° Bio-Lab - I20-1/2/3 and I21-1/2/3 ligations sent sequencing. <partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> resuspended from iGEM 2010 Distribution Kit. Trasformation of pAH123 and <partinfo>BBa_J72008</partinfo> helper into MC1061 and MG1655.
August, 3rd	46° Bio-Lab - <partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> single colony pick from plates. MC1061 and MG1655 colonies transformed with helpers were picked from plates and inoculated and and incubated in proper medium. PCR from colony (MG1655 and MC1061) using synthesized primers to check them and to verify a negative control for integration.
August, 4th	47° Bio-Lab - Glycerol stocks for MC1061 and MG1655 strains transformed with pAH123 or <partinfo>BBa_J72008</partinfo> helper plasmids. Miniprep of MC1061 and MG1655 to check again the presence of helper plasmids. Digestion with SpeI and gel run. We got positive samples but a bad run. So we picked again from plates, this time single colonies.
August, 5th	48° Bio-Lab - Miniprep of newly picked colonies MC1061 and MG1655, digestion with SpeI and gel run.
August, 6th	49° Bio-Lab -
August, 7th	50° Bio-Lab -
August, 8th	51° Bio-Lab -
AUGUST: WEEK 2
August, 9th	52° Bio-Lab -
August, 10th	53° Bio-Lab -
August, 11th	54° Bio-Lab -
August, 12th	55° Bio-Lab -
August, 13th	56° Bio-Lab -
August, 14th	57° Bio-Lab -
August, 15th	58° Bio-Lab -

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 <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana5 #August, 1st|August, 1st]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab - I20 and I21 plates showed colonies. Some were picked, inoculated and grown in proper medium.
 </td></tr>
 <tr><th align="center" width="12%" colspan="2"><b>AUGUST: WEEK 1</b></th></tr>