INSTRUMENTS

Contents 1 AYTOCLAVE 2 BACTERIA ROOM 3 INCUBATOR FOR PLATES 4 INCUBATOR AND SHAKER FOR LIQUID CULTURES 5 MOLECULAR BIOLOGY ROOM 6 HOOD 7 WATER BATH 8 INSTRUMENT FOR ELECTROPHORESIS 9 GEL IMAGE ACQUISITION SYSTEM 10 FREEZER ROOM 11 FRIDGE ROOM 12 TECAN Infinite 2000

AYTOCLAVE

An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C or more, typically for 15 to 20 minutes depending on the size of the load and the contents. It was invented by Charles Chamberland in 1879, although a precursor known as the steam digester was created by Denis Papin in 1679. The name comes from Greek auto, ultimately meaning self, and Latin clavis meaning key — a self-locking device.

BACTERIA ROOM

This is a room in which we work with bacteria. The temperature in this room is very hot and it smells like puke.

INCUBATOR FOR PLATES

This is a oven in which plates are kept at 37 C to grow bacteria.

INCUBATOR AND SHAKER FOR LIQUID CULTURES

This is a oven where bacteria are kept to grow. In this case you can define temperature an rpm.

MOLECULAR BIOLOGY ROOM

This is the room in which we treat DNA.

HOOD

It is a laminar flow hood for handling highly toxic products.

WATER BATH

It is a bath in which water is heated to high temperatures for gel extraction and transformation.

INSTRUMENT FOR ELECTROPHORESIS

Gel electrophoresis apparatus - An agarose gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera.

GEL IMAGE ACQUISITION SYSTEM

It is a UV system with camera used to acquire gel run snapshots.

FREEZER ROOM

This is a room where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria.

FRIDGE ROOM

This is a room in which we have a fridge to stock plates and LB medium

TECAN Infinite 2000

Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. We used this instrument for the following measurement techniques: *fluorescence intensity *absorbance