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Revision as of 14:38, 5 August 2010

July 12th

• Grow more:

  -B1002(terminator)
  -K112808
  -CI promoter
  -LuxR

• Digests:

  -J24679+B1002(D+B) (XP+phosphatase)
  -J24679+B1006(A+C) (XP+phosphatase)
  -C0261 (SP)
  -K274100 (ES)
  -B1002 (EX)
  -B1006 (EX)

• Ligations:

  Insert       Vector

1)J24679+B1002 C0261 2)J24679+B1006 C0261 3)K274100 B1002

                B1006

• Transform:

   -R0051 from kit plate 2009

• Gel order:

  -Ladder
  -B1006
  -B1002
  -K274100 (July 1st)
  -K274100 (June 22nd)
  -C0261

• All the bands showed up in the correct spots and so they were all cut out and gel purified.

July13th

• Colony PCR

 - J24679+B1006+C0261(C) (A-D)
 - J24679+B1006+C0261(A) (A-D)
 - K274100+B1002 (A-D)
 - K274100+B1006 (A-C)
 - J24679+B1002+C0261(D) (A-D)
 - J24679+B1002+C0261 (B)(A-D)

• Digests:

 -R0010+P0451 (ES+phosphatase)
 -R0010 (EX)

• Ligations:

 -R0010+P0451

• Gel Order:

 -Ladder
 -R0051
 -1A-D
 -2A-D
 -5A-D

• Gel Order:

 -Ladder
 -Lux
 -3A-D

 -Ladder
 -4A-C
 -6A-D

The ones that are marked in the picture above tells us that 6A and 6C were grown up.

July 14th

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