Team:UNIPV-Pavia/Calendar/June

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Revision as of 09:16, 5 August 2010

JUNE

Finally Bio-Lab activity began! Select the day you are interested to find out all the details.

DATE	ACTIVITY
June, 3rd	9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
JUNE: WEEK 2
June, 7th	<partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha. Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".
June, 8th	Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.
June, 9th	MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of: I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X) I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
June, 10th	Transformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.
June, 11th	Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).
June, 12th	iGEM EU workshop.
June, 13th	iGEM EU workshop.
JUNE: WEEK 3
June, 14th	6° Bio-Lab - Lorenzo and Nicolò. Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 fro screening.
June, 15th	7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro. MiniPrep, gel run/cut and ligation of I4: <partinfo>BBa_K165037</partinfo> (S-P) + I1 (X-P) I5: I2 (E-S) + <partinfo>BBa_J61001</partinfo> (E-X) I6: I3 (E-S) + <partinfo>BBa_T9002</partinfo> (E-X)
June, 16th	8° Bio-Lab - Susanna Z., Sara and Nicolò. Tranformation of I4 (DH5-alpha), I5 (TOP10) and I6 (DH5-alpha).
June, 17th	9° Bio-Lab - Susanna Z., Nicolò and Riccardo. Colonies were peaked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.
June, 18th	10° Bio-Lab - Susanna Z., Susanna S. and Federica. MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)
JUNE: WEEK 4
June, 21st	11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.
June, 22nd	12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò.
June, 23rd	13° Bio-Lab - Sara, Susanna Z., Nicolò. 10° Meeting - Updating about Bio-Lab activity.
June, 24th	14° Bio-Lab - Sara, Susanna Z., Alessandro.
June, 25th	15° Bio-Lab - Susanna Z., Chiara, Riccardo.
JULY: WEEK 1
June, 28th	16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo.
June, 29th	17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo.
June, 30th	18° Bio-Lab - Susanna Z.

@@ Line 16: / Line 16: @@
 <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
 <tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
-<tr><th align="center" width="12%" colspan="2"><b>WEEK 2</b></th></tr>
+<tr><th align="center" width="12%" colspan="2"><b>JUNE: WEEK 2</b></th></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.<br></td></tr>
@@ Line 28: / Line 28: @@
 <tr><td align="center" valign="top"><html><a name="June_12th"></a></html>June, 12th</td><td align="left">iGEM EU workshop.<br></td></tr>
 <tr><td align="center" valign="top"><html><a name="June_13th"></a></html>June, 13th</td><td align="left">iGEM EU workshop.<br></td></tr>
-<tr><th align="center" width="12%" colspan="2"><b>WEEK 3</b></th></tr>
+<tr><th align="center" width="12%" colspan="2"><b>JUNE: WEEK 3</b></th></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 14th|June, 14th]]</td><td align="left">6° Bio-Lab - Lorenzo and Nicolò.<br> Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 fro screening.</td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 15th|June, 15th]]</td><td align="left">7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro. MiniPrep, gel run/cut and ligation of
@@ Line 37: / Line 37: @@
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 17th|June, 17th]]</td><td align="left">9° Bio-Lab - Susanna Z., Nicolò and Riccardo. Colonies were peaked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 18th|June, 18th]]</td><td align="left">10° Bio-Lab - Susanna Z., Susanna S. and Federica. MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)<br></td></tr>
-<tr><th align="center" width="12%" colspan="2"><b>WEEK 4</b></th></tr>
+<tr><th align="center" width="12%" colspan="2"><b>JUNE: WEEK 4</b></th></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 21st|June, 21st]]</td><td align="left">11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 22nd|June, 22nd]]</td><td align="left">12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò.<br></td></tr>
@@ Line 43: / Line 43: @@
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Sara, Susanna Z., Alessandro.<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Susanna Z., Chiara, Riccardo.<br></td></tr>
-<tr><th align="center" width="12%" colspan="2"><b>WEEK 5</b></th></tr>
+<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 1</b></th></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 28th|June, 28th]]</td><td align="left">16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo.<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 29th|June, 29th]]</td><td align="left">17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo.<br></td></tr>