Team:UNIPV-Pavia/Calendar/June

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<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
<tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
<tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
 +
<tr><th align="center" width="12%" colspan="2"><b>WEEK 2</b></th></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.<br></td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.<br></td></tr>

Revision as of 08:58, 5 August 2010
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JUNE


Finally Bio-Lab activity began! Select the day you are interested to find out all the details.

DATEACTIVITY
June, 3rd9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
WEEK 2
June, 7th<partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha.
Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".
June, 8thInoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.
June, 9thMiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of:
  • I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
  • I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X)
  • I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
  • I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
June, 10thTransformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.
June, 11thInoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).
June, 12thiGEM EU workshop.
June, 13thiGEM EU workshop.
June, 14th6° Bio-Lab - Lorenzo and Nicolò.
Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 fro screening.
June, 15th7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro. MiniPrep, gel run/cut and ligation of
  • I4: <partinfo>BBa_K165037</partinfo> (S-P) + I1 (X-P)
  • I5: I2 (E-S) + <partinfo>BBa_J61001</partinfo> (E-X)
  • I6: I3 (E-S) + <partinfo>BBa_T9002</partinfo> (E-X)
June, 16th8° Bio-Lab - Susanna Z., Sara and Nicolò. Tranformation of I4 (DH5-alpha), I5 (TOP10) and I6 (DH5-alpha).
June, 17th9° Bio-Lab - Susanna Z., Nicolò and Riccardo. Colonies were peaked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.
June, 18th10° Bio-Lab - Susanna Z., Susanna S. and Federica. MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)
June, 21st11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.
June, 22nd12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò.
June, 23rd13° Bio-Lab - Sara, Susanna Z., Nicolò.
10° Meeting - Updating about Bio-Lab activity.
June, 24th14° Bio-Lab - Sara, Susanna Z., Alessandro.
June, 25th15° Bio-Lab - Susanna Z., Chiara, Riccardo.
June, 28th16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo.
June, 29th17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo.
June, 30th18° Bio-Lab - Susanna Z.

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