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Week of 7/12
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | ==July 12th== | ||
| + | |||
| + | |||
| + | *Grow more: | ||
| + | -B1002(terminator) | ||
| + | -K112808 | ||
| + | -CI promoter | ||
| + | -LuxR | ||
| + | |||
| + | |||
| + | *Digests: | ||
| + | -J24679+B1002(D+B) (XP+phosphatase) | ||
| + | -J24679+B1006(A+C) (XP+phosphatase) | ||
| + | -C0261 (SP) | ||
| + | -K274100 (ES) | ||
| + | -B1002 (EX) | ||
| + | -B1006 (EX) | ||
| + | |||
| + | *Ligations: | ||
| + | Insert Vector | ||
| + | 1)J24679+B1002 C0261 | ||
| + | 2)J24679+B1006 C0261 | ||
| + | 3)K274100 B1002 | ||
| + | B1006 | ||
| + | |||
| + | |||
| + | *Transform: | ||
| + | -R0051 from kit plate 2009 | ||
| + | |||
| + | |||
| + | *Gel order: | ||
| + | -Ladder | ||
| + | -B1006 | ||
| + | -B1002 | ||
| + | -K274100 (July 1st) | ||
| + | -K274100 (June 22nd) | ||
| + | -C0261 | ||
<center>[[Image:7-12-10.jpg]]</center> | <center>[[Image:7-12-10.jpg]]</center> | ||
| + | |||
| + | *All the bands showed up in the correct spots and so they were all cut out and gel purified. | ||
| + | |||
| + | |||
| + | ==July13th== | ||
| + | |||
| + | |||
| + | *Colony PCR | ||
| + | - J24679+B1006+C0261(C) (A-D) | ||
| + | - J24679+B1006+C0261(A) (A-D) | ||
| + | - K274100+B1002 (A-D) | ||
| + | - K274100+B1006 (A-C) | ||
| + | - J24679+B1002+C0261(D) (A-D) | ||
| + | - J24679+B1002+C0261 (B)(A-D) | ||
| + | |||
| + | |||
| + | *Digests: | ||
| + | -R0010+P0451 (ES+phosphatase) | ||
| + | -R0010 (EX) | ||
| + | |||
| + | *Ligations: | ||
| + | -R0010+P0451 | ||
| + | |||
| + | |||
| + | *Gel Order: | ||
| + | -Ladder | ||
| + | -R0051 | ||
| + | -1A-D | ||
| + | -2A-D | ||
| + | -5A-D | ||
| Line 5: | Line 72: | ||
| + | |||
| + | *Gel Order: | ||
| + | -Ladder | ||
| + | -Lux | ||
| + | -3A-D | ||
| + | |||
| + | -Ladder | ||
| + | -4A-C | ||
| + | -6A-D | ||
<center>[[Image:7-13-10 001.jpg]]</center> | <center>[[Image:7-13-10 001.jpg]]</center> | ||
| + | The ones that are marked in the picture above tells us that 6A and 6C were grown up. | ||
| + | |||
| + | |||
| + | |||
| + | ==July 14th== | ||
| + | |||
| + | |||
| + | |||
Revision as of 02:22, 5 August 2010
July 12th
- Grow more:
-B1002(terminator) -K112808 -CI promoter -LuxR
- Digests:
-J24679+B1002(D+B) (XP+phosphatase) -J24679+B1006(A+C) (XP+phosphatase) -C0261 (SP) -K274100 (ES) -B1002 (EX) -B1006 (EX)
- Ligations:
Insert Vector
1)J24679+B1002 C0261 2)J24679+B1006 C0261 3)K274100 B1002
B1006
- Transform:
-R0051 from kit plate 2009
- Gel order:
-Ladder -B1006 -B1002 -K274100 (July 1st) -K274100 (June 22nd) -C0261
- All the bands showed up in the correct spots and so they were all cut out and gel purified.
July13th
- Colony PCR
- J24679+B1006+C0261(C) (A-D) - J24679+B1006+C0261(A) (A-D) - K274100+B1002 (A-D) - K274100+B1006 (A-C) - J24679+B1002+C0261(D) (A-D) - J24679+B1002+C0261 (B)(A-D)
- Digests:
-R0010+P0451 (ES+phosphatase) -R0010 (EX)
- Ligations:
-R0010+P0451
- Gel Order:
-Ladder -R0051 -1A-D -2A-D -5A-D
- Gel Order:
-Ladder -Lux -3A-D
-Ladder -4A-C -6A-D
The ones that are marked in the picture above tells us that 6A and 6C were grown up.
July 14th
