Team:Michigan/Protocols

From 2010.igem.org

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==General Outline for Biobrick Manipulations==
 
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==Protocols==
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[[Media:QS Procedures.pdf|Lsr Circuit Test Protocols]]
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==General Outline for Biobrick Manipulations==
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[[Image:Igem_order_of_procedures.jpg|900px]]

Revision as of 18:38, 4 August 2010


Michigan Header




Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Heat shock

Transformation-electroporation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

MODIFIED Miniprep

Nanodrop

DNA Digest

Running a Gel

DNA Purification

Ligation

  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

biofilm assay protocol

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations

Igem order of procedures.jpg

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