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Week of 6/21
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| + | ==June 21== | ||
| + | |||
| + | Sent to the sequencing center: | ||
| + | Base Pairs | ||
| + | 1)C3RFP 681 | ||
| + | 2)K091146 1266 | ||
| + | 3)K112022 1499 | ||
| + | 4)K145150 66 | ||
| + | 5)R0063 151 | ||
| + | 6)R1062 56 | ||
| + | 7)K081007 796 | ||
| + | |||
| + | *We made 1 liter of LB media. | ||
| + | |||
| + | |||
| + | ==June 22== | ||
| + | |||
| + | *Flasks Minipreped: | ||
| + | -J23108 | ||
| + | -J23116 | ||
| + | -J37033 | ||
| + | -K091107 | ||
| + | -C0261 | ||
| + | -K274100 | ||
| + | -C3RFP | ||
| + | -K112808 | ||
| + | |||
| + | *All the minipreps were cut with the following enzymes: | ||
| + | -J23108 (SP) | ||
| + | -J23116 (SP) | ||
| + | -J37033 (XP + phosphatase) | ||
| + | -K091107(SP) | ||
| + | -C0261 (XP + phosphatase) | ||
| + | -K274100(XP + phosphatase) | ||
| + | -C3RFP (EX) | ||
| + | -K112808(ES + phosphatase) | ||
| + | |||
| + | |||
| + | *Ligations: | ||
| + | - J23108 + J37033 | ||
| + | - J23116 + J37033 | ||
| + | - K091107 + C0261 | ||
| + | - K091107 + K274100 | ||
| + | - C3RFP + K112808 | ||
| + | |||
| + | *Transformations: | ||
| + | - B0010 Plate 1 Well 13D | ||
| + | - R0010 Plate 1 Well 1D | ||
| + | - K081007 Plate 2 Well 10J | ||
| + | - J24679 Plate 1 Well 7L | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | *We also did a PCR on K081007 + R0010, J23116 + J37033, and J23108 + J37033. | ||
| + | |||
<center>[[Image:6-22-10.jpg]]</center> | <center>[[Image:6-22-10.jpg]]</center> | ||
| + | |||
| + | |||
<center>[[Image:6-22-10 001.jpg]]</center> | <center>[[Image:6-22-10 001.jpg]]</center> | ||
| + | |||
| + | |||
| + | |||
| + | ==June 23== | ||
| + | |||
| + | *Transform today: | ||
| + | - R0061 Plate 1 Well 14P | ||
| + | - R0062 Plate 1 Well 6O | ||
| + | - R0063 Plate 1 Well 8A | ||
| + | - K091146 Plate 2 Well 8H | ||
| + | |||
| + | *Digests: | ||
| + | - K091107 (SP) | ||
| + | - R0010 (SP) | ||
| + | - J24679 (ES) | ||
| + | - J23108 (SP) | ||
| + | - J23116 (SP) | ||
| + | - CO261 (XP) | ||
| + | - K081107 (XP) | ||
| + | - B0010 (EX) | ||
| + | - J37033 (XP) | ||
| + | - K274100 (XP) | ||
| + | |||
| + | *Sent to get sequenced: | ||
| + | 1) R0010 | ||
| + | 2) B0010 | ||
| + | 3) K081107 | ||
| + | 4) J24679 | ||
| + | |||
| + | |||
| + | ==June 24== | ||
| + | |||
| + | Gel Order: | ||
| + | 1) Ladder | ||
| + | 2) J24679 | ||
| + | 3) K091107 | ||
| + | 4) J37033 | ||
| + | 5) J23108 | ||
| + | 6) K274100 | ||
| + | 7) B0010 | ||
| + | 8) R0010 | ||
| + | 9) K081107 | ||
| + | 10)CO261 | ||
| + | 11)J23116 | ||
<center>[[Image:6-24-10.jpg]]</center> | <center>[[Image:6-24-10.jpg]]</center> | ||
| + | |||
| + | RE-DIGEST: | ||
| + | - CO261 | ||
| + | - K081107 | ||
| + | |||
| + | RE-Transform: | ||
| + | - J37033 | ||
| + | - K091107 | ||
| + | |||
| + | *Sent to sequencing: | ||
| + | 1) R0061 | ||
| + | 2) R0062 | ||
| + | 3) R0063 | ||
| + | 4) K091146 | ||
| + | |||
| + | |||
| + | ==June 25== | ||
| + | |||
| + | *Sent to sequencing: | ||
| + | 1) K091107 | ||
| + | 2) J37033 | ||
| + | |||
| + | |||
| + | *Digests: | ||
| + | - K091107 (SP) | ||
| + | - J23108 (SP) | ||
| + | - J23116 (SP) | ||
| + | - CO261 (XP) | ||
| + | - R0010 (SP) | ||
| + | - K274100 (XP) | ||
| + | - J37033 (XP) | ||
| + | - J24679 (ES) | ||
| + | - K081007 (XP) | ||
| + | |||
| + | *Ligations: | ||
| + | - K091107 + K274100 | ||
| + | - J23108 + J37033 | ||
| + | - J23116 + J37033 | ||
| + | - C0261 + J24679 | ||
| + | - R0010 + K081007 | ||
| + | |||
| + | The 4 that were sent to get sequenced yesterday were ran on a gel. The gel order is provided below: | ||
| + | |||
| + | - Ladder | ||
| + | - K091146 | ||
| + | - R0061 | ||
| + | - R0062 | ||
| + | - R0063 | ||
<center>[[Image:6-25-10.jpg]]</center> | <center>[[Image:6-25-10.jpg]]</center> | ||
Revision as of 15:33, 4 August 2010
Contents |
June 21
Sent to the sequencing center:
Base Pairs 1)C3RFP 681 2)K091146 1266 3)K112022 1499 4)K145150 66 5)R0063 151 6)R1062 56 7)K081007 796
- We made 1 liter of LB media.
June 22
- Flasks Minipreped:
-J23108 -J23116 -J37033 -K091107 -C0261 -K274100 -C3RFP -K112808
- All the minipreps were cut with the following enzymes:
-J23108 (SP) -J23116 (SP) -J37033 (XP + phosphatase) -K091107(SP) -C0261 (XP + phosphatase) -K274100(XP + phosphatase) -C3RFP (EX) -K112808(ES + phosphatase)
- Ligations:
- J23108 + J37033 - J23116 + J37033 - K091107 + C0261 - K091107 + K274100 - C3RFP + K112808
- Transformations:
- B0010 Plate 1 Well 13D - R0010 Plate 1 Well 1D - K081007 Plate 2 Well 10J - J24679 Plate 1 Well 7L
- We also did a PCR on K081007 + R0010, J23116 + J37033, and J23108 + J37033.
June 23
- Transform today:
- R0061 Plate 1 Well 14P - R0062 Plate 1 Well 6O - R0063 Plate 1 Well 8A - K091146 Plate 2 Well 8H
- Digests:
- K091107 (SP) - R0010 (SP) - J24679 (ES) - J23108 (SP) - J23116 (SP) - CO261 (XP) - K081107 (XP) - B0010 (EX) - J37033 (XP) - K274100 (XP)
- Sent to get sequenced:
1) R0010 2) B0010 3) K081107 4) J24679
June 24
Gel Order:
1) Ladder 2) J24679 3) K091107 4) J37033 5) J23108 6) K274100 7) B0010 8) R0010 9) K081107 10)CO261 11)J23116
RE-DIGEST:
- CO261 - K081107
RE-Transform:
- J37033 - K091107
- Sent to sequencing:
1) R0061 2) R0062 3) R0063 4) K091146
June 25
- Sent to sequencing:
1) K091107 2) J37033
- Digests:
- K091107 (SP) - J23108 (SP) - J23116 (SP) - CO261 (XP) - R0010 (SP) - K274100 (XP) - J37033 (XP) - J24679 (ES) - K081007 (XP)
- Ligations:
- K091107 + K274100 - J23108 + J37033 - J23116 + J37033 - C0261 + J24679 - R0010 + K081007
The 4 that were sent to get sequenced yesterday were ran on a gel. The gel order is provided below:
- Ladder - K091146 - R0061 - R0062 - R0063
