"
Team:Harvard/fences/parts
From 2010.igem.org
(Difference between revisions)
| Line 47: | Line 47: | ||
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr> | <tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr> | ||
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr> | <tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr> | ||
| - | <tr><td> | + | <tr><tdExp2MutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CCT ACA CAC GGA ACT CCT GGA AAT TAA GAC CG-3'</td></tr> |
| - | + | <tr><td>Exp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CGG TCT TAA TTT CCA GGA ACT CCG TGT GTA GG-3'</td></tr> | |
<tr><td>Bam.Gal4.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr> | <tr><td>Bam.Gal4.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr> | ||
<tr><td>HmRXR.Hind.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr> | <tr><td>HmRXR.Hind.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr> | ||
Revision as of 18:44, 3 August 2010
parts and primers
Vectors
| Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
| V1 | TEXT | INSERT TEXT HERE | Kanamycin | pat | |
| V2 | TEXT | INSERT TEXT HERE | Kanamycin | nptII | |
| V3 | TEXT | INSERT TEXT HERE | Kanamycin | pat | |
| V4 | TEXT | INSERT TEXT HERE | Kanamycin | nptII | |
| V5 | TEXT | INSERT TEXT HERE | Kanamycin | nptII |
Primers
| Name | Description | Sequence |
| Arp2mutForward | Point mutagenesis to remove restriction enzyme site | 5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3' |
| Arp2mutReverse | Point mutagenesis to remove restriction enzyme site | 5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3' |
| Point mutagenesis to remove restriction enzyme site | 5'-CCT ACA CAC GGA ACT CCT GGA AAT TAA GAC CG-3' | |
| Exp2mutReverse | Point mutagenesis to remove restriction enzyme site | 5'-CGG TCT TAA TTT CCA GGA ACT CCG TGT GTA GG-3' |
| Bam.Gal4.Fwd | INSERT DESCRIPTION HERE | 5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3' |
| HmRXR.Hind.Rev | INSERT DESCRIPTION HERE | 5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3' |
| LCRXR | INSERT DESCRIPTION HERE | 5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3' |
| VP16.Hind.Fwd | INSERT DESCRIPTION HERE | 5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3' |
| VP16.Rev | INSERT DESCRIPTION HERE | 5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3' |
| NLS.serine.BB.Fwd | INSERT DESCRIPTION HERE | 5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3' |
| NLS.serine.BB.Rev | INSERT DESCRIPTION HERE | 5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3' |
| Fwd.BB.AtEXP2 | for amplification/extraction of an arabidopsis germination-specific promoter | 5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3' |
| Rev.BB.AtEXP2 | for amplification/extraction of an arabidopsis germination-specific promoter | 5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3' |
| Fwd.BB.AtARP2 | for amplification/extraction of the arabidopsis ARP2 promoter | 5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3' |
| Rev.BB.AtARP2 | for amplification/extraction of the arabidopsis ARP2 promoter | 5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3' |
| barnase2.BB.fwd | addition of biobrick sites/ends to barnase gene | 5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3' |
| BB.Barnase.Rev | addition of biobrick sites/ends to barnase gene | 5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3' |
| NLS.BB.Fwd | addition of biobrick sites/ends to the arabidopsis NLS | 5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3' |
| NLS.BB.Rev | addition of biobrick sites/ends to the arabidopsis NLS | 5'/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA-3' |
| LacIn.BB.Fwd | INSERT DESCRIPTION HERE | 5'-CCT TGA ATT CGC GGC CGC ATC TAG AAT GAA AC AGT AAC GTT ATA CGA TGT C-3' |
| LacIN.BB.Rev | INSERT DESCRIPTION HERE | 5' AAG GTC GCA GCA GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA CTG CCC GCT TTC CAG TCG GGA AA-3' |
| BB.Gal3DBD.Fwd | INSERT DESCRIPTION HERE | 5'-CTT TTC TAG AAT GAA GCT ACT GTC TTC TAT C-3' |
| BB.Gal4DBD.Rev | INSERT DESCRIPTION HERE | 5'-AAG GCT GCA GCG GCC GCT ACT AGT CGA TAC AGT CAA CTG TCT TTG-3' |
| BB.Barstar.Fwd | addition of biobrick sites/ends to barstar gene | 5'CCT TTC TAG AAT GAA AAA AGC AGT CAT TAA C-3' |
| BB.Barstar.Rev | addition of biobrick sites/ends to barstar gene | 5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA AGA AAG TATA GAT GGT GAT GTC-3' |
| 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | ||
| VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
| VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |
