Team:Harvard/fences/parts
From 2010.igem.org
(Difference between revisions)
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<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr> | <tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr> | ||
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr> | <tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr> | ||
- | <tr><td> | + | <tr><td>Bam.Gal4.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr> |
- | <tr><td> | + | <tr><td>HmRXR.Hind.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr> |
- | <tr><td> | + | <tr><td>LCRXR</td><td>INSERT DESCRIPTION HERE</td><td>5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'</td></tr> |
- | <tr><td> | + | <tr><td>VP16.Hind.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'</td></tr> |
- | <tr><td> | + | <tr><td>VP16.REV</td><td>5'- CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'</td><td></td></tr> |
- | <tr><td> | + | <tr><td>NLS.serine.BB.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr> |
- | <tr><td> | + | <tr><td>NLS.serine.BB.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'</td></tr> |
- | <tr><td> | + | <tr><td>Fwd.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3'</td></tr> |
- | <tr><td> | + | <tr><td>Rev.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'</td></tr> |
- | <tr><td> | + | <tr><td>Fwd.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'</td></tr> |
<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | <tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr> | ||
<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> | <tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr> |
Revision as of 18:13, 3 August 2010
![](https://static.igem.org/mediawiki/2010/a/a5/Harvard2010fencebanner.png)
parts and primers
Vectors
Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
V1 | TEXT | INSERT TEXT HERE | Kanamycin | pat | |
V2 | TEXT | INSERT TEXT HERE | Kanamycin | nptII | |
V3 | TEXT | INSERT TEXT HERE | Kanamycin | pat | |
V4 | TEXT | INSERT TEXT HERE | Kanamycin | nptII | |
V5 | TEXT | INSERT TEXT HERE | Kanamycin | nptII |
Primers
Name | Description | Sequence |
Arp2mutForward | Point mutagenesis to remove restriction enzyme site | 5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3' |
Arp2mutReverse | Point mutagenesis to remove restriction enzyme site | 5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3' |
Bam.Gal4.Fwd | INSERT DESCRIPTION HERE | 5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3' |
HmRXR.Hind.Rev | INSERT DESCRIPTION HERE | 5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3' |
LCRXR | INSERT DESCRIPTION HERE | 5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3' |
VP16.Hind.Fwd | INSERT DESCRIPTION HERE | 5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3' |
VP16.REV | 5'- CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3' | |
NLS.serine.BB.Fwd | INSERT DESCRIPTION HERE | 5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3' |
NLS.serine.BB.Rev | INSERT DESCRIPTION HERE | 5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3' |
Fwd.BB.AtEXP2 | for amplification/extraction of an arabidopsis germination-specific promoter | 5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3' |
Rev.BB.AtEXP2 | for amplification/extraction of an arabidopsis germination-specific promoter | 5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3' |
Fwd.BB.AtARP2 | for amplification/extraction of the arabidopsis ARP2 promoter | 5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3' |
VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |