Team:Stockholm/26 July 2010

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(→Sequencing CPP transportan 10 version C)
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=== LMWP C and N ===
=== LMWP C and N ===
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::'''Plasmid prep and glycerol stock'''
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Plasmid prep and glycerol stock
 
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid
-
 
+
::'''DNA concentrations'''
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'''DNA concentrations'''
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::{|border="1" cellspacing="0"  
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{|border="1" cellspacing="0" cellpadding="1"
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! Sample  
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|'''Sample'''
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! DNA (ng/μl)  
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|'''DNA (ng/μl)'''
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|-
|-
|LMWP N   
|LMWP N   
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|}
|}
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=== over-expression in pEX - remaking the comassie-gel ===
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-
MIX:
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# 4ml  LB
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=== over-expression in pEX ===
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# 40µl glycose (1%)
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::'''- remaking the comassie-gel'''
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# 8µl  Amp (0,5%)
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-
Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
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 +
{|border="1" cellspacing="0"
 +
! Mix
 +
|
 +
|-
 +
| LB  
 +
| 4ml
 +
|-
 +
| glycose  
 +
| 40µl (1%)
 +
|-
 +
| Amp
 +
| 8µl (0.5%)
 +
|}
 +
 
 +
 
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*Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
----
----
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==Nina==
==Nina==

Revision as of 14:59, 3 August 2010

Contents

Mimmi

LMWP C and N

Plasmid prep and glycerol stock


The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid

DNA concentrations
Sample DNA (ng/μl)
LMWP N 60
LMWP C 80


over-expression in pEX

- remaking the comassie-gel


Mix
LB 4ml
glycose 40µl (1%)
Amp 8µl (0.5%)


  • Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON

Nina

Sequencing CPP transportan 10 version C

I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.

  • 15 ul vector
  • 1.5 ul 10uM VR2 primer

9: ASB003N X99

40: ASB003N X98

Inoculation of IgG protease

I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep.

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