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Team:TU Delft/23 June 2010 content
From 2010.igem.org
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| - | [[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight [https://2010.igem.org/Team:TU_Delft#page=pages/blog | + | [[Team:TU_Delft/protocols/ligation|Ligations]] were performed using the overnight [https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=22_June_2010 digested BioBricks]. The following ligation reactions was performed: |
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Revision as of 12:28, 3 August 2010
Lab work
Characterization of Anderson RBS sequences
Ligations were performed using the overnight digested BioBricks. The following ligation reactions was performed:
| # | BioBrick | Fragment 1 | Fragment 2 | Recipient vector |
| 1 | 4 μL μL ‘E–J1100–S’ | 3 μL μL ‘X–I10341–P’ | 1.5 μL ‘E–linear pSB1T3–P’ | |
| 2 | 4 μL μL ‘E–J1101–S’ | 3 μL μL ‘X–I10341–P’ | 1.5 μL ‘E–linear pSB1T3–P’ | |
| 3 | 4 μL μL ‘E–J1107–S’ | 3 μL μL ‘X–I10341–P’ | 1.5 μL ‘E–linear pSB1T3–P’ | |
| 4 | 4 μL μL ‘E–J1117–S’ | 3 μL μL ‘X–I10341–P’ | 1.5 μL ‘E–linear pSB1T3–P’ | |
| 5 | 4 μL μL ‘E–J1127–S’ | 3 μL μL ‘X–I10341–P’ | 1.5 μL ‘E-linear pSB1T3–P’ | |
| 6 | negative control | - | 3 μL μL ‘X–I10341–P’ | 1.5 μL ‘E–linear pSB1T3–P’ |
We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The mixtures were incubated overnight at 16 °C.
