"
Team:TU Delft/18 June 2010 content
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | =Cleaning= | |
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy. | After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy. | ||
We also booked a hotel in Boston! | We also booked a hotel in Boston! | ||
| - | + | =Lab work= | |
| - | + | ==Emulsifier== | |
Pieter worked on the emulsifying activity assay. Four samples were tested: | Pieter worked on the emulsifying activity assay. Four samples were tested: | ||
Revision as of 19:17, 2 August 2010
Cleaning
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
We also booked a hotel in Boston!
Lab work
Emulsifier
Pieter worked on the emulsifying activity assay. Four samples were tested:
1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
| # | Amount of SDS (%) | Hexalkane (mL) |
| 1 | 0 | 0 |
| 2 | 0 | 0.02 |
| 3 | 0.01 | 0.02 |
| 4 | 1 | 0.02 |
2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.
3. Shake 1 hour with 200 strokes/min at 30°C
4. Measure absorbance OD600
| # | A600 |
| 1 | 0 |
| 2 | 0 |
| 3 | 0.4 |
| 4 | 0.2 |
