From 2010.igem.org
(Difference between revisions)
|
|
| Line 60: |
Line 60: |
| | |- | | |- |
| | |} | | |} |
| | + | |
| | + | Plates were let grow ON at 37°C. |
| | | | |
| | ==August, 3rd== | | ==August, 3rd== |
Revision as of 15:32, 2 August 2010
|
|
|
|
|
AUGUST: WEEK 1
August, 2nd
Miniprep and quantification at Nanodrop of:
- I20-1: 98,2 ng/ul
- I20-2: 63,6 ng/ul
- I20-3: 41,5 ng/ul
- I21-1: 45 ng/ul
- I21-2: 45 ng/ul
- I21-3: 54 ng/ul
These samples were prepared and sent (400ng) to BMR Genomics for sequencing.
From iGEM 2010 Distribution Kit were resuspended the following parts:
- <partinfo>BBa_R0062</partinfo> (pLux; Plate 1, Well 6O)
- <partinfo>BBa_K081008</partinfo> (RBS + lasI; Plate 2, Well 10N)
both in vector <partinfo>pSB1A2</partinfo>.
Transformation (1ul) of the following parts (resuspended/miniprepped):
| Part | Strain
|
| pAH123 | MC1061
|
| MG1655
|
| <partinfo>BBa_J72008</partinfo> | MC1061
|
| MG1655
|
| <partinfo>BBa_R0062</partinfo> | DH5-alpha
|
| <partinfo>BBa_K081008</partinfo> | DH5-alpha
|
Transformed cells were plated on proper LB+Amp agar plate:
| Part | Plate resistance
|
| pAH123 | Amp 50 ug/ml
|
| <partinfo>BBa_J72008</partinfo>
|
| <partinfo>BBa_R0062</partinfo> | Amp 100 ug/ml
|
| <partinfo>BBa_K081008</partinfo>
|
Plates were let grow ON at 37°C.
August, 3rd
August, 4th
August, 5th
August, 6th
August, 7th
August, 8th
|
|
|
"
