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Team:UNIPV-Pavia/Calendar/August/settimana1
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<html><p align="center"><font size="4"><b>AUGUST: WEEK 1</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>AUGUST: WEEK 1</b></font></p></html><hr><br> | ||
==August, 2nd== | ==August, 2nd== | ||
| + | Miniprep and quantification at Nanodrop of: | ||
| + | *I20-1: 98,2 ng/ul | ||
| + | *I20-2: 63,6 ng/ul | ||
| + | *I20-3: 41,5 ng/ul | ||
| + | *I21-1: 45 ng/ul | ||
| + | *I21-2: 45 ng/ul | ||
| + | *I21-3: 54 ng/ul | ||
| + | These samples were prepared and sent (400ng) to BMR Genomics for sequencing. | ||
| + | From iGEM 2010 Distribution Kit were resuspended the following parts: | ||
| + | *<partinfo>BBa_R0062</partinfo> (pLux; Plate 1, Well 6O) | ||
| + | *<partinfo>BBa_K081008</partinfo> (RBS + lasI; Plate 2, Well 10N) | ||
| + | both in vector <partinfo>pSB1A2</partinfo>. | ||
| + | |||
| + | Transformation (1ul) of the following parts (resuspended/miniprepped): | ||
| + | {|border='1' align='center' | ||
| + | |- | ||
| + | |'''Part''' || '''Strain''' | ||
| + | |- | ||
| + | |pAH123 || MC1061 | ||
| + | |- | ||
| + | |pAH123 || MG1655 | ||
| + | |- | ||
| + | |<partinfo>BBa_J72008</partinfo> || MC1061 | ||
| + | |- | ||
| + | |<partinfo>BBa_J72008</partinfo> || MG1655 | ||
| + | |- | ||
| + | |<partinfo>BBa_R0062</partinfo> || DH5-alpha | ||
| + | |- | ||
| + | |<partinfo>BBa_K081008</partinfo> || DH5-alpha | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | Transformed cells were plated on proper LB+Amp agar plate | ||
| + | {|border='1' align='center' | ||
| + | |- | ||
| + | |'''Part''' || '''Plate resistance''' | ||
| + | |- | ||
| + | |pAH123 || Amp 50 ug/ml | ||
| + | |- | ||
| + | |pAH123 || Amp 50 ug/ml | ||
| + | |- | ||
| + | |<partinfo>BBa_J72008</partinfo> || Amp 50 ug/ml | ||
| + | |- | ||
| + | |<partinfo>BBa_J72008</partinfo> || Amp 50 ug/ml | ||
| + | |- | ||
| + | |<partinfo>BBa_R0062</partinfo> || Amp 100 ug/ml | ||
| + | |- | ||
| + | |<partinfo>BBa_K081008</partinfo> || Amp 100 ug/ml | ||
| + | |- | ||
| + | |} | ||
==August, 3rd== | ==August, 3rd== | ||
Revision as of 15:26, 2 August 2010
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