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Team:Newcastle/5 July 2010
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(New page: ==LacI BioBrick Construction== ===Aims=== *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1A...)
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(New page: ==LacI BioBrick Construction== ===Aims=== *To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1A...)
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Revision as of 10:52, 2 August 2010
Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Refrigerated transformation plates
Protocol
- Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3
Inference
- Transformed Ecoli DH5alpha grown up overnight to create a large stock of plasmid ready for extraction
