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HKU-Hong Kong/13 July 2010
From 2010.igem.org
(Difference between revisions)
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Different combinations of enzymes were used for the isolation. | Different combinations of enzymes were used for the isolation. | ||
=Result= | =Result= | ||
| - | [[ | + | [[Image:13jul.jpg|500px|results of digestions]] |
| + | Controls were run in parallel to the plasmid after various treatments. | ||
| + | An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments. | ||
| + | The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent. | ||
Revision as of 08:16, 31 July 2010
13 July 2010:Con't BBa_k112808 isolation
Different combinations of enzymes were used for the isolation.
Result
Controls were run in parallel to the plasmid after various treatments.
An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments.
The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent.
