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Team:Michigan/Protocols
From 2010.igem.org
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[[Media:Protocol_Heat_Shock_Transformation_2.pdf|Transformation-heat shock]] | [[Media:Protocol_Heat_Shock_Transformation_2.pdf|Transformation-heat shock]] | ||
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*Getting parts from the 360 well registry plates | *Getting parts from the 360 well registry plates | ||
*Competent cell preparation | *Competent cell preparation | ||
Revision as of 02:20, 31 July 2010
General Outline for Biobrick Manipulations
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Epifluorescence Microscope Usage - H.H. Dow
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
