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	[[Media:Quorum+Sensing+Tables-1-.pdf|Logic Table]]		[[Media:Quorum+Sensing+Tables-1-.pdf|Logic Table]]
-	[[Media:QS_Procedures-1-.pdf|Lsr Circuit Test Protocols]]	+	[[Media:QS Procedures.pdf|Lsr Circuit Test Protocols]]

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Revision as of 17:56, 30 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol

Group-Specific Protocols

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols