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Team:Harvard/vectors/parts
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Revision as of 19:54, 29 July 2010
abstract lab notebook agrobacteria and plant engineering meet the vectors plant transformation parts & primers references
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parts and primers
Vectors
For more information on the vectors visit our "meet the vectors" page| Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
| V1 | pORE Open Series 1 (O1) | Agrobacterium vector open series | Kanamycin | pat | |
| V2 | pORE Open Series 2 (O2) | Agrobacterium vector open series | Kanamycin | nptII | |
| V3 | pORE Expression Series 3 (E3) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | pat | |
| V4 | pORE Expression Series 4 (E4) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | nptII | |
| V5 | pORE Reporter Series 1 (R1) | Agrobacterium vector with gusA reporter | Kanamycin | nptII | |
| V6 | pORE Reporter Series 3 (R3) | Agrobacterium vector with smgfp reporter | Kanamycin | nptII | |
| V7 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | ||
| V8 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | ||
| V9 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | ||
| V10 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | ||
| V11 | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | ||
| V12 | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII |
Primers
| Name | Description | Sequence |
| VP3 | HindIII-pENTCUP2 forward primer for Expression Series insert | |
| VP4 | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | |
| VP5 | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
| VP6 | gusA-NheI reverse primer for Reporter Series 1 insert | |
| VP7 | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
| VP8 | smGFP-NheI reverse primer for Reporter Series 1 insert | |
| VP9 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP11 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP12 | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP13 | 2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP14 | 3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP15 | 4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
| VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |
