Team:Newcastle/Nanodrop

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(New page: ==Nanodrop== ===Materials required=== # Water # Buffer used to resuspend the sample # Nanodrop machine # Pipettes ===Procedures=== # Select Nanodrop program from the desktop # To clean ...)
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Revision as of 14:43, 28 July 2010

Nanodrop

Materials required

  1. Water
  2. Buffer used to resuspend the sample
  3. Nanodrop machine
  4. Pipettes

Procedures

  1. Select Nanodrop program from the desktop
  2. To clean Nanodrop, add a drop of water on the spectrometer and press blank
  3. After cleaning, wipe the water off
  4. To equalizen the equipment, add 3 μl of the buffer used in the sample and press blank
  5. Wipe the buffer off
  6. To measure sample, add 3 μl of the sample and press measure
  7. If dealing with multiple samples, clean the equipment with water at regular intervals
  8. After measurement, clean the equipment with a drop of water on the spectrometer and press blank