Team:ETHZ Basel/Biology
From 2010.igem.org
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As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721). | As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721). | ||
- | Currently we are working on putting all the BioBricks into a storage vector. The image | + | Currently we are working on putting all the BioBricks into a storage vector. The image [[Image:Constructs.pdf|200px|thumb|left|alt text]] shows all the constructs we plan to clone. |
Revision as of 10:52, 28 July 2010
As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).
Currently we are working on putting all the BioBricks into a storage vector. The image File:Constructs.pdf shows all the constructs we plan to clone.