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	As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).		As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).
-	Currently we are working on putting all the BioBricks into a storage vector. The image ([[Image:Constructs.jpg]]) shows all the constructs we plan to clone.	+	Currently we are working on putting all the BioBricks into a storage vector. The image ([[Image:Construct.jpg|thumb|alt=A cartoon centipede reads books and types on a laptop.|The Wikipede edits ''[[Myriapoda]]''.]]) shows all the constructs we plan to clone.

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Revision as of 09:28, 28 July 2010

As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).

Currently we are working on putting all the BioBricks into a storage vector. The image () shows all the constructs we plan to clone.