Team:Michigan/Quorum Sensing
From 2010.igem.org
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*MDAI2: '''0.855''' | *MDAI2: '''0.855''' | ||
Need to start culture with OD .02 | Need to start culture with OD .02 | ||
- | * .02 / .873 = 2.29% | + | * .02 / .873 = '''2.29%''' |
- | * .02 / .855 = 2.34% | + | * .02 / .855 = '''2.34%''' |
Obtained 40% glucose solution from Ann - Lin Lab | Obtained 40% glucose solution from Ann - Lin Lab | ||
*need .8% glc final conc. | *need .8% glc final conc. | ||
- | * .8/ 40 = 2% | + | * .8/ 40 = '''2%''' |
Started 100mL culture of W3110 in 500mL flask | Started 100mL culture of W3110 in 500mL flask | ||
-2.29 mL W3110 culture | -2.29 mL W3110 culture | ||
-2 mL 40% glucose | -2 mL 40% glucose | ||
-95.7 mL LB | -95.7 mL LB | ||
- | **100 - 2.29 - 2 = 95.71 | + | **100 - 2.29 - 2 = '''95.71''' |
Started 100mL culture of MDAI2 in 500mL flask | Started 100mL culture of MDAI2 in 500mL flask | ||
-2.34 mL MDAI2 culture | -2.34 mL MDAI2 culture | ||
-2 mL 40% glucose | -2 mL 40% glucose | ||
-95.7 mL LB | -95.7 mL LB | ||
- | **100 - 2.34 - 2 = 95.66 | + | **100 - 2.34 - 2 = '''95.66''' |
*placed both cultures in 37°C, 225 rpm shaking - ERB 1230, '''11:50am''' | *placed both cultures in 37°C, 225 rpm shaking - ERB 1230, '''11:50am''' | ||
--[[User:Infekt|Infekt]] 23:19, 27 July 2010 (UTC) | --[[User:Infekt|Infekt]] 23:19, 27 July 2010 (UTC) |
Revision as of 23:20, 27 July 2010