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Revision as of 15:04, 27 July 2010 (view source) Jcnossen (Talk | contribs) ← Older edit		Revision as of 18:34, 27 July 2010 (view source) Jcnossen (Talk | contribs) (→RBS Characterization) Newer edit →
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		+	Next step: We want to relate the RBS sequence to the strength, so it might be possible to say predict something about the other Anderson RBS sequences.

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Revision as of 18:34, 27 July 2010

Alkane degradation

Yesterday's digestion products were checked on a gel.

RBS Characterization

For our RBS characterization project, 5 different RBS sequences from the [http://partsregistry.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Anderson Anderson RBS family] where placed in front of GFP and measured over 18 hours using a Gen5 fluorenscence and absorbance plate reader

Strength was calculated by taking the mean of the ratio between the expression of known RBS ([http://partsregistry.org/Part:BBa_B0032 B0032]) and expression Anderson RBS over some time. Expression being the measured fluorescence divided by measured biomass (absorbance, OD).

The measurements where taken from the part of the curve where optimal growth can be assumed, so from 0:40 until 2:30

RBS	Strength
[http://partsregistry.org/Part:BBa_J61100 J61100]	0.047513
[http://partsregistry.org/Part:BBa_J61101 J61101]	0.119831
[http://partsregistry.org/Part:BBa_J61107 J61107]	0.065454
[http://partsregistry.org/Part:BBa_J61117 J61117]	0.038518
[http://partsregistry.org/Part:BBa_J61127 J61127]	0.087334
[http://partsregistry.org/Part:BBa_B0032 B0032]	0.300000

Next step: We want to relate the RBS sequence to the strength, so it might be possible to say predict something about the other Anderson RBS sequences.