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From 2010.igem.org

Revision as of 23:05, 25 July 2010 (view source) Evolver (Talk | contribs) ← Older edit		Revision as of 23:19, 25 July 2010 (view source) Evolver (Talk | contribs) Newer edit →
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	[[Image:biobrickgel.jpg|300px]]		[[Image:biobrickgel.jpg|300px]]
-	[[Image:ladder.jpg|300px]]	+	[[Image:ladder.jpg|100px]]
		+
		+	lane 1 - ladder
		+
		+	lane 2 - INP NC sample 1 (K265008)
		+
		+	lane 3 - INP NC sample 2 (K265008)
		+
		+	lane 4 - T7 GFPmut3b (I719015)
		+
		+	lane 5 - T7 Promoter (I719005)
		+
		+	lane 6 - T7 RNA polymerase (K145001)
		+
		+	lane 7 - pLsrA-YFP (K117008)
		+
		+	lane 8 - LsrA promoter (K117002)
		+
		+	lane 9 - OmpA (K103006)

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Revision as of 23:19, 25 July 2010

7/10/2010 13:00

• digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1.

7/13/2010 9:00

• inoculate competent cell culture

• harvest I719015, K145001, K117008, K117002, K103006 from registry

7/15/2010 4:30

• streak plate INP NC sample

7/18/2010 5:00

• miniprep INP NC sample

7/21/2010 6:00

• digest INP NC with EcoRI and SpeI

7/23/2010 9:00

• gel electrophoresis of all biobricks

lane 1 - ladder

lane 2 - INP NC sample 1 (K265008)

lane 3 - INP NC sample 2 (K265008)

lane 4 - T7 GFPmut3b (I719015)

lane 5 - T7 Promoter (I719005)

lane 6 - T7 RNA polymerase (K145001)

lane 7 - pLsrA-YFP (K117008)

lane 8 - LsrA promoter (K117002)

lane 9 - OmpA (K103006)