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Team:Stockholm/22 July 2010
From 2010.igem.org
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== Hassan == | == Hassan == | ||
| - | tyrp1 | + | *tyrp1 |
| - | nalp1 | + | *nalp1 |
| - | mitf | + | *mitf |
| - | CGRP | + | *CGRP |
| - | S100B | + | *S100B |
| - | foxd3 | + | *foxd3 |
| - | GGA1 | + | *GGA1 |
| - | LMP7 | + | *LMP7 |
| - | TAPBP | + | *TAPBP |
==Johan== | ==Johan== | ||
Revision as of 23:16, 25 July 2010
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Contents |
Hassan
- tyrp1
- nalp1
- mitf
- CGRP
- S100B
- foxd3
- GGA1
- LMP7
- TAPBP
Johan
PCR
The PCR samples that was run on a gel to verify that the colonies had been successfully site-directed mutagenesised seemed to be lost, and a new colony PCR was performed to verify the site-directed mutagenesis before cleaving and adding it to the target vectors.
The ladder is FastRuler DNA ladder low range, but the bands does not show the expected size. Did I mistakenly use the FastRuler DNA ladder middle range?
