From 2010.igem.org
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| - | |[[Image:UNIPV10_screening_ligations_I11-I16.jpg|thumb|300px|center|Screening ligations I11-I16]] || [[Image:UNIPV10_creening_ligations_I17-I19_I7-8-104C5.jpg|thumb|300px|center|Screening ligations I17-I19, I7/I8/I104C5]] | + | |[[Image:UNIPV10_screening_ligations_I11-I16.jpg|thumb|300px|center|Screening ligations I11-I16]] || [[Image:UNIPV10_creening_ligations_I17-I19_I7-8-104C5.jpg|thumb|300px|center|Screening ligations I17-I19, I7/I8/I10-4C5]] |
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Revision as of 15:39, 25 July 2010
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JULY: WEEK 3
July, 12th
 Gel run/cut of phasins amplified via PCR July, 13th
July, 14th
 Quality control for HELPER and CRIM plasmids July, 15th
 Quality control for pBHR68 and CRIM plasmids July, 16th
 Screening ligations I11-I16 |  Screening ligations I17-I19, I7/I8/I10-4C5 |
Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it.
We called it "THE RING" (RING) and we built it from our I5 (Cm resistance - <partinfo>BBa_P1004</partinfo> - + TT - <partinfo>BBa_B0015</partinfo> - + R6K ori - <partinfo>BBa_J61001</partinfo>) in <partinfo>pSB1A2</partinfo>:
- Digestion of I5 XbaI-PstI
- Gel extraction
- Quantification with Nanodrop (15ng/ul)
- Ligation of X and P sites each other (used 2ul of DNA)
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