From 2010.igem.org
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| | ==July, 15th== | | ==July, 15th== |
| - | [[Image:UNIPV10_1_QC_CRIM_pBHR68.jpg|thumb|200px|center|Quality control for pBHR68 and CRIM plasmids]] | + | [[Image:UNIPV10_QC_CRIM_pBHR68.jpg|thumb|200px|center|Quality control for pBHR68 and CRIM plasmids]] |
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| | ==July, 16th== | | ==July, 16th== |
Revision as of 12:10, 24 July 2010
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JULY: WEEK 3
July, 12th
 Gel run/cut of phasins amplified via PCR July, 13th
July, 14th
 Quality control for HELPER and CRIM plasmids July, 15th
July, 16th
 Screening ligations I11-I16  Screening ligations I17-I19, I7/I8/I104C5 Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it.
We called it "THE RING" (RING) and we built it from our I5 (Cm resistance - <partinfo>BBa_P1004</partinfo> - + TT - <partinfo>BBa_B0015</partinfo> - + R6K ori - <partinfo>BBa_J61001</partinfo>) in <partinfo>pSB1A2</partinfo>:
- Digestion of I5 XbaI-PstI
- Gel extraction
- Quantification with Nanodrop (15ng/ul)
- Ligation of X and P sites each other (used 2ul of DNA)
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