Team:Cambridge/Materials

Materials available

• T-shirt funding = £400 (they do make a difference! Polo shirts are the way to go)
• + extra £200 with logos on T shirt
• Publicity: down to us. BluSci, Varsity. Horizons. Naked Scientist. NewScientist? Radio 4.
• Up profile at the Jamboree
• Sterilin have sponsored us, we need to write an article for Sterilin Leader, "Sterilin supports Cambridge iGEM Team". Who we are? Why are we doing it, what are we using for Sterilin. Assignment: Bill and Ben (the flowerpot men) Relevant "Happy Birthday Sterilin" in luciferin? for Autumn edition need by November-ish or earlier

• Partners information:
  • Sterilin
  • Starlab (2 P2s and 1 P10 for Gibson assembly)
  • Cambridge BioSciences (free Zymoresearch equip)
  • Qiagen
    • Kit for eluting in smaller volume available for trial?
  • DNA 2.0
    • Long turn-around time, need to subclone into vector for registry
  • invitrogen
    • lending of transilluminator
    • gels
      • EX gels = 10 mins, use wisely, contain CyberSafe)
      • also some gels which contain EtBr (use for diagnostics, not purification), each gel has a specific program
      • clonewell gels
  • Bioline
    • Ladders
    • Testing "velocity"?
  • Fermentas
    • Restriction enzymes / ligases
  • GRI
    • G storm over summer
      • How easy is it to use?
      • Send an email to plant sciences saying "Would you like to see our machines? Demonstration models"
  • BMG Labtech
  • Clonetech
    • Trial out dried down PCR reactions. Just add (deionised) water, primers, and template in PCR tube strips
  • VWR
    • Primer synthesis vouchers for oligonucleotide synthesis and peptide synthesis
  • Gene Services
    • £100 worth of DNA sequencing, collection in Genetics. We must supply plasmids and primers (none in stock?) = 10 ds sequences (required for registry)
  • (Visual/Fisher?) Scientific
  • Labtech
    • Nanodrop loan
  • Anachem
    • Earth Saver pipette tips (biodegradable) N.B. Boxes recyclable.
    • We're helping the environment
  • New England Biologicals
    • Provided Gibson Assembly equipment.
    • Phusion, DNTp, Gel loading dyes, gel loading ladders, T5 exonuclease, phusion master mix
    • 100 reactions worth
  • Duncan's freezer has antibiotics, DNA ladders, PCR-grade water
  • CamBio
    • T5 exonuclease
  • Biolegio
    • Free oligo-synthesis up to v. long nucleotides (150+) £250 worth. Useful for combining promoter and RBC? Additionals at 5p per base.
  • Eppendorff
    • Thermo mixer shaker, will shake and incubate E.coli cultures
  • BibiScientific
    • PCR machine?
  • Biosilta
    • Growth medium with carbon source locked up in slow-release pellet = linear growth phase
    • Specialist 96-cell plates for high throughput
  • Thermofissure Scientific
    • Supplying food and drink after talk in Cambridge
• Bought equipment
  • Bio-rad freeze and squeeze columns - cut out block of agarose containing DNA. Freeze in a freezer. When solid run through column, by the end DNA will come into bottom. Test for Duncan Size cut-off?
  • Specialist filters for drop dialysis
  • Jim's lab has a robot! (for high through-put work)