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July 3rd Week

July 19th

We performed a miniprep in order to purify the plasmid XXXXXXXXX, that was kindly sent by Kausik Li, containing the Aplysia prion XXXXXXXX.

The digestion with BamH1 clearly showed that the purification had been successful. (PHOTO needed).

We also started the protocol for next day's transformation of yeast strain 2606 with the plasmid pLZ, which we already tried last week but obtained no results.

July 20th

The transformation conducted the day before showed different results. The 5523 strain of Saccharomyces cerevisiae grew fine. However, the 2606 strain showed no growth at all. This may be due to the excesive OD of the culture at the beginning of the transformation process (so the yeasts were not at exponential growth phase).

Therefore we repeated the transformation protocol of the 2606 (with LiAc) strain and let the yeast grow at 30ºC during three days.

BREAKING STORIES: Due to maintenance issues, the air condicioned and the regular devices were off from 3 p.m. on. So we had to find the lab machines plugged in to the emergency system and we found out the true meaning of the word HOT. More soon, on this underground section known as BREAKING STORIES.