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NanoDrop Spectrophotometer
From 2010.igem.org
(Difference between revisions)
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| - | Measure the concentration and purity of extracted DNA using absorbance (automated nanodrop machine) | + | Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!) |
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| + | The ideal concentration of DNA is 150 ng/ml! | ||
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| + | == Method: == | ||
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#Log onto computer, open program ND 1000 | #Log onto computer, open program ND 1000 | ||
#Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program | #Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program | ||
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#reblank about every 10 samples | #reblank about every 10 samples | ||
#blank with water at end, wipe and log off | #blank with water at end, wipe and log off | ||
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Revision as of 08:39, 22 July 2010
Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)
The ideal concentration of DNA is 150 ng/ml!
Method:
- Log onto computer, open program ND 1000
- Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
- Wipe, apply EBor appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.
- Wipe, apply sample to nib, measure
- reblank about every 10 samples
- blank with water at end, wipe and log off
