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Team:TU Delft/21 July 2010 content
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| + | Unfortunately there were no transformants on [https://2010.igem.org/Team:TU_Delft#/blog?blog=20_July_2010 yesterday's] plates. This is most likely due to the fact that the ligation didn't work with the small pieces of DNA of the RBSs. Next plan is to cut open the RBS plasmid without removing the RBS (with SpeI and PstI) and insert the gene in this plasmid. We will try this tomorrow. | ||
Revision as of 14:27, 21 July 2010
Alkane degradation
Unfortunately there were no transformants on yesterday's plates. This is most likely due to the fact that the ligation didn't work with the small pieces of DNA of the RBSs. Next plan is to cut open the RBS plasmid without removing the RBS (with SpeI and PstI) and insert the gene in this plasmid. We will try this tomorrow.
