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Team:Calgary/20 July 2010
From 2010.igem.org
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[[Image:07.20.2010. Raida PCR BBK primer.jpg|thumb|400px|Gel electrophoresis of Raida's PCR products: BBK primers that anneal before the multiple cloning sites. Lane 4 and 5 : Lux0047 A + B0015 psB1AK3 Lane 6: J23002 + Lux0047E, Lane7: R0040 + I3502, Lane 8: Lux0047E, Lane 9: Master Mix]] | [[Image:07.20.2010. Raida PCR BBK primer.jpg|thumb|400px|Gel electrophoresis of Raida's PCR products: BBK primers that anneal before the multiple cloning sites. Lane 4 and 5 : Lux0047 A + B0015 psB1AK3 Lane 6: J23002 + Lux0047E, Lane7: R0040 + I3502, Lane 8: Lux0047E, Lane 9: Master Mix]] | ||
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<u> Raida </u> | <u> Raida </u> | ||
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As we can see in the gel electrophoresis image, the expected results has been obtained. For example, Lane 8 ladder contains only the Lux0047E gene where as Lane 6 has J23002 + Lux0047E, hence the band in Lane 8 goes slightly further relative to Lane 6 because it is a shorter band. Lanes 4 and 5 show exactly the same band because they are the same genes in the same plasmid backbone. Furthermore, Lane 9 shows no band because it is the Master Mix and our negative control. Therefore, it can be concluded that the '''primers are functional.''' | As we can see in the gel electrophoresis image, the expected results has been obtained. For example, Lane 8 ladder contains only the Lux0047E gene where as Lane 6 has J23002 + Lux0047E, hence the band in Lane 8 goes slightly further relative to Lane 6 because it is a shorter band. Lanes 4 and 5 show exactly the same band because they are the same genes in the same plasmid backbone. Furthermore, Lane 9 shows no band because it is the Master Mix and our negative control. Therefore, it can be concluded that the '''primers are functional.''' | ||
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<u> Jeremy </u> | <u> Jeremy </u> | ||
Today I completed the ligation and transformation of the I0500+E0034 plasmid switch. I also ran the PCR products and the results came back negative. So I set up another PCR and Gel. | Today I completed the ligation and transformation of the I0500+E0034 plasmid switch. I also ran the PCR products and the results came back negative. So I set up another PCR and Gel. | ||
| + | <u>Patrick</u> | ||
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| + | Today I helped plasmid prep the ''degP'' promoter and ''cpxR'' promoter on a GFP and RFP reporter device each. I also started looking to literature regarding high and low-copy plasmids for the modelling project. | ||
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Revision as of 22:03, 20 July 2010
Tuesday July 20, 2010
Gel electrophoresis of Raida's PCR products: BBK primers that anneal before the multiple cloning sites. Lane 4 and 5 : Lux0047 A + B0015 psB1AK3 Lane 6: J23002 + Lux0047E, Lane7: R0040 + I3502, Lane 8: Lux0047E, Lane 9: Master Mix
Raida
Today I set up and ran two 1% gels: one for my PCR products from yesterday and the second one for Emily's PCR products- both of which were done to test whether our primers are working or not. Please see the gel to the side: As we can see in the gel electrophoresis image, the expected results has been obtained. For example, Lane 8 ladder contains only the Lux0047E gene where as Lane 6 has J23002 + Lux0047E, hence the band in Lane 8 goes slightly further relative to Lane 6 because it is a shorter band. Lanes 4 and 5 show exactly the same band because they are the same genes in the same plasmid backbone. Furthermore, Lane 9 shows no band because it is the Master Mix and our negative control. Therefore, it can be concluded that the primers are functional.
Jeremy
Today I completed the ligation and transformation of the I0500+E0034 plasmid switch. I also ran the PCR products and the results came back negative. So I set up another PCR and Gel.
Patrick
Today I helped plasmid prep the degP promoter and cpxR promoter on a GFP and RFP reporter device each. I also started looking to literature regarding high and low-copy plasmids for the modelling project.
No notebook page exists for this date. Sorry!
