"
Team:Stanford/Notebook/Lab Work/Week 5
From 2010.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
*normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am) | *normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am) | ||
| + | |||
| + | ==7/20 Tuesday== | ||
| + | ===Laura's Lab Notebook=== | ||
| + | *helped Alex with preparation of competent cells | ||
| + | *see protocol: [http://openwetware.org/wiki/Stanford/BIOE44:Module_1:Day3 Preparing Electrocompetent Cells] | ||
| + | |||
Revision as of 18:23, 20 July 2010
|
Spring: Brainstorming | Spring Meetings
Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries
|
7/19 Monday
Laura's Notebook
set up the following ligations from the gel extractions done by me, Karina, and Francisco on Friday, 7/14/10)
- Francisco ran and imaged diagnostic gel on Friday
| Ligation Recipe | |
|---|---|
| dH2O | none |
| vector (with terminators attached) | 5.0 uL |
| insert (RFP or GFP) | 12.0 uL |
| 10X buffer | 2.0 uL |
| T4 ligase | 1.0 uL |
- normally run for 10 minutes at room temperature, overnight this time (started at 11:30 am)
7/20 Tuesday
Laura's Lab Notebook
- helped Alex with preparation of competent cells
- see protocol: [http://openwetware.org/wiki/Stanford/BIOE44:Module_1:Day3 Preparing Electrocompetent Cells]
Nanodrop data (deemed unreliable based on 260/230, possibly due do residual EtBr contamination)
| part | 260/280 | 260/230 | ng/uL |
| vector/terminator | 1.77 | 0.03 | 19.1 |
| RFP | 1.86 | 0.02 | 16.2 |
| GFP | 1.83 | 0.02 | 14.8 |
