Team:Stanford/Notebook/Lab Work/Week 4

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries

7/12 Monday

Christopher's Notebook

• Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
• Gel Extract
• Run Gel of Gel Extracted Digest Products from Friday:

 3C5     E/P 2738 bp (buffer 3)
F2620   E/S (on pSB1A2) 1061 bp

• I746908 E/P (on psb1A2) 2093 bp-can’t separate
• B0034 X/P (on psb1A2) 12 bp-do NOT run on gel

• J23100 E/S (on psb1A2) 35 bp-do NOT run on gel

7/13 Tuesday

Christopher's Notebook

• Run Gels (0.8%) of Restriction Digests:

 1M, 2M, 2J (X/P)
 F2620 (E/S)
 3C5 (E/P)
 I0500 (E/S)

Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation.

Laura's Notebook

• restreaked plates from 7/1/10 (with Karina)
• for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
• plate ID's:

7/14 Wednesday

Laura's Notebook

• plates restreaked yesterday look good- bacteria grew well, some individual colonies
• helped Francisco with minipreps (Promega kit) of : terminator, GFP, RFP
  • Francisco set up liquid cultures yesterday afternoon
• Francisco poured, ran 0.8% gel
  • yesterday's digestions, with today's minipreps as controls
• gel extraction: (I cut out bands, Francisco and Karina extracted)