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Team:Stanford/Notebook/Lab Work/Week 4
From 2010.igem.org
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*Run Gels (0.8%) of Restriction Digests: | *Run Gels (0.8%) of Restriction Digests: | ||
1M, 2M, 2J (X/P) | 1M, 2M, 2J (X/P) | ||
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'''Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation'''. | '''Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation'''. | ||
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===Laura's Lab Notebook=== | ===Laura's Lab Notebook=== | ||
Revision as of 17:39, 20 July 2010
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Spring: Brainstorming | Spring Meetings
Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries
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7/12 Monday
Christopher's iGEM Notebook
- Run Gel of Restricted Digested RBS+GFP+Terminators (I746908 partial)
- Gel Extract
- Run Gel of Gel Extracted Digest Products from Friday:
3C5 E/P 2738 bp (buffer 3) F2620 E/S (on pSB1A2) 1061 bp
- I746908 E/P (on psb1A2) 2093 bp-can’t separate
- B0034 X/P (on psb1A2) 12 bp-do NOT run on gel
- J23100 E/S (on psb1A2) 35 bp-do NOT run on gel
7/13 Tuesday
Christopher's Notebook
- Run Gels (0.8%) of Restriction Digests:
1M, 2M, 2J (X/P) F2620 (E/S) 3C5 (E/P) I0500 (E/S)
Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation.
Laura's Lab Notebook
- restreaked plates from 7/1/10 (with Karina)
- for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
- plate ID's:
