Team:UNIPV-Pavia/Calendar/July/settimana4

JULY: WEEK 4

July, 19th

Trasformation of RING into:

• BW25141 (pir+)
• BW25142 (pir116)
• BW23474 (pir116)
• DH5alpha
• MG1655

Cultures were plated on:

• BW2514: Cm 34ug/ml
• BW25142: Cm 34ug/ml
• BW234741: Cm 34ug/ml
• DH5alpha: Cm 12,5ug/ml
• MG1655: Cm 12,5ug/ml

July, 20th

Results for plates incubated ON, 37° C:

• BW23474 (pir116): showed colonies
• BW25141 (pir+): showed colonies
• BW25142 (pir116): showed colonies
• DH5alpha: didn't show colonies
• MG1655: didn't show colonies (even if there are a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).

Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.

July, 21st

July, 22nd

July, 23rd