Team:Stanford/Notebook/Lab Work/Week 1
From 2010.igem.org
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**Finalize sRNA library | **Finalize sRNA library | ||
**9kb plasmid | **9kb plasmid | ||
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*Meeting scheudle | *Meeting scheudle | ||
**Monday morning is good for logistics | **Monday morning is good for logistics |
Revision as of 21:26, 19 July 2010
June 28
General Meeting
- Wiki stuff
- Everyone should sign up on 2010.igem.org website
- Take advantage of the new calendar system
- Need to transfer current website over
- Current wiki needs a new vision
- Francisco + Alex assembling wiki committee
- Everyone should sign up on 2010.igem.org website
- Transform the first wave of cells
- Pick colonies
- Colony PCR
- Sequence parts to validate
- Use the Smolke sequencing network
- Characterize promoters
- Digest, ligate
- Need to acquire enzymes
- Need an PO account.
- Email Jeanne
- Get competent cells
- Getting DNA
- Assembly PCR
- PCR from host cells
- Synthesize de novo
- RNA team
- Use RNAstructure
- Maybe use ViennaRNA or dinamelt
- Talk to Arkin and Berkeley Postdocs
- Finalize sRNA library
- 9kb plasmid
- Use RNAstructure
- Meeting scheudle
- Monday morning is good for logistics
- Group meetings for presentations
- One person presents their work in the context of everything else
- Journal club
- Karina will set up a doodle