"
Team:Stanford/NotebookPage/8 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{Template:Stanford_Main_Menu_0910}} {{Template: Stanford Notebook Calendar}} <div style="width:720px;text-align:justify"> '''Christopher's Notebook Section''' *'''Running a Gel of F2620, ...) |
Karpadillo (Talk | contribs) |
||
| Line 28: | Line 28: | ||
| - | + | ||
| + | ==Laura's Lab notebook== | ||
| + | miniprepped Alex's/Chris' liquid cultures, using Promega kit | ||
| + | *2 each of: | ||
| + | **psb1K3 | ||
| + | **J23107 | ||
| + | **B0032 | ||
| + | *eluted in 100 uL H2O | ||
| + | |||
| + | {| | ||
| + | !Nanodrop Data | ||
| + | |- | ||
| + | | tube ID || 260/280 || 260/230 || ng/uL | ||
| + | |- | ||
| + | |psB1K3-1 || 1.77 || 1.08 || 71.3 | ||
| + | |- | ||
| + | |psB1K3-2 || 1.73 || 1.01 || 77.8 | ||
| + | |- | ||
| + | |J23107-1 || 1.87 || 1.60 || 116.3 | ||
| + | |- | ||
| + | |J23107-2 || 1.90 || 1.74 || 142.0 | ||
| + | |- | ||
| + | |B0032-1 || 1.81 || 1.20 || 68.1 | ||
| + | |- | ||
| + | |B0032-2 || 1.83 || 1.32 || 72.5 | ||
| + | |} | ||
Latest revision as of 20:59, 19 July 2010
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Christopher's Notebook Section
- Running a Gel of F2620, pBAD, and psb4A5:
- Make a 0.8% agarose gel (0.4 g of agarose in 50 ml of TAE)
- Heat in microwave until agarose is completely dissolved
- Add 10.0 ul of EtBr to mix (5000x for a 50 ml solution)
- let run for approximately 1 hr
- refer below for bands
4A5 E/P 3395 bp
I0500 E/S (on psb2K3) 1210 bp
F2620 E/S (on pSB1A2) 1061 bp
Gel Extract the Fragments Running a Gel of PCR Product: -first need to PCR cleanup -then run on 1.4% agarose gel (0.7 g of agarose in 50 ml of TAE) -this is necessary because of the 800 bp size of the fragment
Miniprep Ligations -F2620+E0240+psb1A2 -pBAD+E0240+psb1A2
Restriction Digests PCR product F2620
Laura's Lab notebook
miniprepped Alex's/Chris' liquid cultures, using Promega kit
- 2 each of:
- psb1K3
- J23107
- B0032
- eluted in 100 uL H2O
| Nanodrop Data | |||
|---|---|---|---|
| tube ID | 260/280 | 260/230 | ng/uL |
| psB1K3-1 | 1.77 | 1.08 | 71.3 |
| psB1K3-2 | 1.73 | 1.01 | 77.8 |
| J23107-1 | 1.87 | 1.60 | 116.3 |
| J23107-2 | 1.90 | 1.74 | 142.0 |
| B0032-1 | 1.81 | 1.20 | 68.1 |
| B0032-2 | 1.83 | 1.32 | 72.5 |
