Team:EPF Lausanne/Protocols

From 2010.igem.org

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Revision as of 13:55, 19 July 2010

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Adapt pH
Agarose Gel
Agar plates production
Autoclave
Electrophoresis
Growing Asaia (Effie)
Ligation
Measuring DNA concentration using NanoDrop
Miniprep (?)
Optical Density (OD)
PCR
Purification --> purification Kit
Transformation of competent cells
UV Imaging
Using the plate reader (Solange)

Media Recipes

Syntax: convention if you want to upload a new protocol

When uploading a file it please rename it in the following way:  prot_yourProcedureName.yourExtension.

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 *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ]
 *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
-* Asaia Maxiprep (effie)
+* Growing Asaia (Effie)
-* Asaia Miniprep (effie)
 *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
 *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
+* Miniprep (?)
 *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
 *[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR ]