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Revision as of 10:16, 19 July 2010 (view source) Yessa (Talk | contribs) (→Summary) ← Older edit		Revision as of 10:30, 19 July 2010 (view source) Yessa (Talk | contribs) (→Summary) Newer edit →
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	#*double digests with Pst1 and Xba1		#*double digests with Pst1 and Xba1
	#Gel electrophoresis		#Gel electrophoresis
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		+	Set up liquid broth culture in LB

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Revision as of 10:30, 19 July 2010

Summary

Screening for insert

1. Miniprep
2. Restriction digests
   • Single digest with Pst1
   • double digests with Pst1 and Xba1
3. Gel electrophoresis

Set up liquid broth culture in LB