"
Team:TU Delft/18 June 2010 content
From 2010.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | ==Cleaning== | ||
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy. | After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy. | ||
We also booked a hotel in Boston! | We also booked a hotel in Boston! | ||
| + | ==Lab work== | ||
| - | + | <h4>Emulsifier</h4> | |
| - | + | ||
| - | + | ||
Pieter worked on the emulsifying activity assay. Four samples were tested: | Pieter worked on the emulsifying activity assay. Four samples were tested: | ||
| Line 39: | Line 39: | ||
4. Measure absorbance OD600 | 4. Measure absorbance OD600 | ||
| - | |||
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
Revision as of 17:32, 17 July 2010
Cleaning
After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
We also booked a hotel in Boston!
Lab work
Emulsifier
Pieter worked on the emulsifying activity assay. Four samples were tested:
1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.
| Sample | Amount of SDS (%) | Hexalkane (mL) |
| 1 | 0 | 0 |
| 2 | 0 | 0.02 |
| 3 | 0.01 | 0.02 |
| 4 | 1 | 0.02 |
2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.
3. Shake 1 hour with 200 strokes/min at 30°C
4. Measure absorbance OD600
| Sample | A600 |
| 1 | 0 |
| 2 | 0 |
| 3 | 0.4 |
| 4 | 0.2 |
