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Team:Alberta/Plates
From 2010.igem.org
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:9) 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate | :9) 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate | ||
:10) L.B.O. stick was incubated overnight at 37C | :10) L.B.O. stick was incubated overnight at 37C | ||
| + | |||
| + | Observations: | ||
| + | : - although the knife cut through the LB agar easily, it was difficult to produce a completely flat surface | ||
| + | : - solidified LB agar was effectively raised and lowered using the dial | ||
| + | : - after incubation, a bacterial lawn was observed and a distinct E.coli smell was present | ||
| + | |||
| + | |||
| + | ==29-06-10== | ||
| + | </p> | ||
| + | :1) Approximately 5mm was sliced off the top of the used LB agar with an ethanol-sterilized knife | ||
| + | :2) Streaked green colony from a plate of Cambridge parts | ||
| + | :3) L.B.O. stick was incubated overnight at 37C | ||
| + | |||
| + | Observations: | ||
| + | : - dark green streak and individual colonies present | ||
| + | : - distinct E.coli smell | ||
Revision as of 18:41, 16 July 2010
| Notebook | Building Parts | Testing Parts | Assembly Method | Competent Cells | Plates | Kit Manual | Software |
|---|
Plates
L.B.O.
24-06-10
Creation of "L.B.O.": a deodorant stick of LB agar
All of the following steps were performed in a safety cabinet under sterile conditions.
- 1) disassembled a Degree deodorant stick and soaked in ethanol
- 2) removed the raising platform and covered it with Parafilm
- 3) coated the insides of the tube with mineral oil
- 5) removed platform and poured LB agar into the base of the stick, waited until it solidified
- 6) the Parafilmed platform was put back into the stick and lowered maximally
- 7) LB agar was poured on top of the platform, until the tube was full, waited until it solidifed
- 8) The top of the LB agar was sliced off with an ethanol-sterilized knife
- 9) 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate
- 10) L.B.O. stick was incubated overnight at 37C
- - although the knife cut through the LB agar easily, it was difficult to produce a completely flat surface
- - solidified LB agar was effectively raised and lowered using the dial
- - after incubation, a bacterial lawn was observed and a distinct E.coli smell was present
29-06-10
- 1) Approximately 5mm was sliced off the top of the used LB agar with an ethanol-sterilized knife
- 2) Streaked green colony from a plate of Cambridge parts
- 3) L.B.O. stick was incubated overnight at 37C
Observations:
- - dark green streak and individual colonies present
- - distinct E.coli smell
