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Team:Alberta/Plates
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| + | == Plates == | ||
| + | |||
| + | __notoc__ | ||
| + | |||
| + | == L.B.O. == | ||
| + | ==24-06-10== | ||
| + | |||
| + | <p>Creation of "L.B.O.": a deodorant stick of LB agar | ||
| + | </p> | ||
| + | <p> | ||
| + | All of the following steps were performed in a safety cabinet under sterile conditions. | ||
| + | :1μL disassembled a Degree deodorant stick and soaked in ethanol | ||
| + | :2μL covered the raising platform with Parafilm | ||
| + | :3μL coated the insides of the tube with mineral oil | ||
| + | :5μL removed platform and poured LB agar into the base of the stick, waited until it solidified | ||
| + | :6μL the Parafilmed platform was put back into the stick and lowered maximally | ||
| + | :7μL LB agar was poured on top of the platform, until the tube was full, waited until it solidifed | ||
| + | :8μL The top of the LB agar was sliced off with an ethanol-sterilized knife | ||
| + | :9μL 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate | ||
| + | :10μL L.B.O. stick was incubated overnight at 37C | ||
Revision as of 18:25, 16 July 2010
| Notebook | Building Parts | Testing Parts | Assembly Method | Competent Cells | Plates | Kit Manual | Software |
|---|
Plates
L.B.O.
24-06-10
Creation of "L.B.O.": a deodorant stick of LB agar
All of the following steps were performed in a safety cabinet under sterile conditions.
- 1μL disassembled a Degree deodorant stick and soaked in ethanol
- 2μL covered the raising platform with Parafilm
- 3μL coated the insides of the tube with mineral oil
- 5μL removed platform and poured LB agar into the base of the stick, waited until it solidified
- 6μL the Parafilmed platform was put back into the stick and lowered maximally
- 7μL LB agar was poured on top of the platform, until the tube was full, waited until it solidifed
- 8μL The top of the LB agar was sliced off with an ethanol-sterilized knife
- 9μL 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate
- 10μL L.B.O. stick was incubated overnight at 37C
