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Team:Davidson-MissouriW
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| - | + | As in the past, the Davidson/Missouri Western multidisciplinary team is using synthetic biology to address a mathematical problem using Escherichia coli. This year, we are addressing the Knapsack Problem, an NP-complete problem that asks, “Given a finite number of weighted items, can one use these items to completely fill a knapsack of fixed capacity?” | |
| - | |[[Image:Davidson-MissouriW_team.png|right|frame| | + | |
| + | Weighted items will be represented by versions of TetA genes that confer measurably distinct levels of tetracycline resistance. We have altered the codons of the wild type TetA gene, optimizing and de-optimizing several segments of the coding sequence. Each TetA variant is coupled with a distinctive fluorescent gene, and each pair is flanked by lox sites. In the presence of Cre protein, the lox mechanism either inverts or excises the coding sequence, yielding different combinations of expressed TetA variants. An expressed variant corresponds to an item being placed in the knapsack. Over-expression of TetA results in cell death. Under-expression of TetA causes the cells to stop growing due to tetracycline in the growth medium. Surviving cells correspond to cells within a certain range of TetA production and the fluorescence tag allows for comparative measurement within this range. | ||
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| + | The team is also working to develop software tools relevant to the specific project and applicable to projects of the wider synthetic biology community. | ||
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| + | |[[Image:Davidson-MissouriW_team.png|right|frame|400px|Davidson/Missouri Western Team]] | ||
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|align="center"|[[Team:Davidson-MissouriW | Team Example]] | |align="center"|[[Team:Davidson-MissouriW | Team Example]] | ||
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<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
Revision as of 21:37, 15 July 2010
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As in the past, the Davidson/Missouri Western multidisciplinary team is using synthetic biology to address a mathematical problem using Escherichia coli. This year, we are addressing the Knapsack Problem, an NP-complete problem that asks, “Given a finite number of weighted items, can one use these items to completely fill a knapsack of fixed capacity?” Weighted items will be represented by versions of TetA genes that confer measurably distinct levels of tetracycline resistance. We have altered the codons of the wild type TetA gene, optimizing and de-optimizing several segments of the coding sequence. Each TetA variant is coupled with a distinctive fluorescent gene, and each pair is flanked by lox sites. In the presence of Cre protein, the lox mechanism either inverts or excises the coding sequence, yielding different combinations of expressed TetA variants. An expressed variant corresponds to an item being placed in the knapsack. Over-expression of TetA results in cell death. Under-expression of TetA causes the cells to stop growing due to tetracycline in the growth medium. Surviving cells correspond to cells within a certain range of TetA production and the fluorescence tag allows for comparative measurement within this range. The team is also working to develop software tools relevant to the specific project and applicable to projects of the wider synthetic biology community. | |
 Davidson/Missouri Western Team | |
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