Team:EPF Lausanne/Protocols

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Revision as of 16:57, 15 July 2010

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Agarose Gel protocol
OD protocol
Autoclave protocol
Agar plates production protocol
adapt ph
electrophoresis
UV imaging
Measuring DNA concentration using NanoDrop
PCR
Using the plate reader
Production of (agar) plates
Asaia Maxiprep
Asaia Miniprep

Media Recipes

Syntax: convention if you want to upload a new protocol

When uploading a file it please rename it in the following way:  prot_yourProcedureName.yourExtension.

@@ Line 10: / Line 10: @@
 * [https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
 * PCR
+*
 * Using the plate reader
 * Production of (agar) plates
@@ Line 21: / Line 22: @@
 *[https://static.igem.org/mediawiki/2010/0/0e/Prot_gly_agar.pdf Gly agar medium]
 *[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
 <b>Syntax: </b>convention if you want to upload a new protocol  <br\>
   When uploading a file it please rename it in the following way: <i> prot_yourProcedureName.yourExtension</i>.