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Team:Cambridge/Quiescence Notes
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If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working. | If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working. | ||
| + | |||
| + | Input from James | ||
| + | * Has to be done in E.Coli, will not work in non Coliform bacteria such as Bacilli. | ||
| + | * Switch on cell division would be very useful, however there are IP issues which must be discussed with David Summers. | ||
| + | * Another constraint is that liquid broth not agar must be used. | ||
| + | * Nobody has looked at it from a microfluidics point of view. | ||
| + | * When done before, the lambda phage takes 3 hours to initiate quiescence after the temperature change. | ||
| + | * The Lambda phage is the most stable to have an off switch, it does not express itself when needed (?) | ||
| + | * The RNA has a very long half life, it would be hard to strip away the system and put it under a new promotor, but, if possible would be extremely useful | ||
Revision as of 15:33, 15 July 2010
- [http://mic.sgmjournals.org/cgi/reprint/155/8/2676.pdf The role of FIS in the Rcd checkpoint and stable maintenance of plasmid ColE1] ie good as the toggle control thingy
- [http://www3.interscience.wiley.com/cgi-bin/fulltext/119127730/PDFSTART Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability]
- [http://aem.asm.org/cgi/reprint/65/6/2710.pdf The quiescent-cell expression system for protein synthesis in Escherichia coli]
- [http://apps.isiknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=12&SID=W11BA3C7bKeHC5PbfoE&page=1&doc=1&colname=WOS ColE1 multimer formation triggers inhibition of Escherichia coli cell division.]
- [http://books.google.com/books?hl=en&lr=&id=OKn60-HZisUC&oi=fnd&pg=PA19&dq=hn-s+nucleoid+bacillus&ots=rstjtFpQ2Z&sig=15uulXPnHz-yyXMvMKRrTXcLk3U#v=onepage&q=h-ns&f=false book about bac genomes]
- [http://www.faqs.org/patents/app/20090004700 D. Summers' Patent Application for Chem. Induction of Quiescence in Bacteria]
Literally no documentation of h-ns and rcd in bacillus.
If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working.
Input from James
- Has to be done in E.Coli, will not work in non Coliform bacteria such as Bacilli.
- Switch on cell division would be very useful, however there are IP issues which must be discussed with David Summers.
- Another constraint is that liquid broth not agar must be used.
- Nobody has looked at it from a microfluidics point of view.
- When done before, the lambda phage takes 3 hours to initiate quiescence after the temperature change.
- The Lambda phage is the most stable to have an off switch, it does not express itself when needed (?)
- The RNA has a very long half life, it would be hard to strip away the system and put it under a new promotor, but, if possible would be extremely useful
